Structure-function studies of the large subunit of ribonucleotide reductase from Escherichia coli.
نویسندگان
چکیده
منابع مشابه
Escherichia coli ribonucleotide reductase expression is cell cycle regulated.
The expression of the genes encoding ribonucleotide reductase in Escherichia coli was investigated in cultures synchronized by obtaining the smallest cells in a population after sucrose gradient centrifugation. Specific activity of ribonucleotide reductase and DNA initiation were found to increase in parallel, periodically as a function of the cell cycle. The expression of nrd was also determin...
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We report on a protein kinase function encoded by the unique N terminus of the herpes simplex virus type 1 (HSV-1) ribonucleotide reductase large subunit (R1). R1 expressed in Escherichia coli exhibited autophosphorylation activity in a reaction which depended on the presence of the unique N terminus. When the N terminus was separately expressed in E. coli and partially purified, a similar auto...
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An iron-sensitive mutant of E. coli with a Mudl phage insertion in the nrdB gene lacks subunit B2 of the key enzyme of DNA synthesis, ribonucleotide reductase. Nevertheless, these cells are capable of growing in minimal media under anaerobic conditions, indicating a second enzyme or pathway for deoxyribonucleotide synthesis. We here show that ribonucleotide reduction cannot be unambiguously mea...
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The nucleotide sequence of the nrdA gene of Escherichia coli. coding for the large subunit of ribonucleotide reductase, was originally published by Carlson et al. (1). However, the published nucleotide sequence of the nrdA gene is afflicted with scattered errors leading to stretches of frame-shifts, which have severe consequences for the deduced protein sequence. The difference in amino acid co...
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Ribonucleotide reductases (EC 1 .I 7.4.1 and EC 1.17.4.2) catalyse the reduction of ribonucleoside 5’-diphosphates or triphosphates to the corresponding 2’-deoxyribonucleotides and are key enzymes for the onset of DNA replication during the cell cycle (Reichard, 1968; Follmann, 1974). The reduction of the 2’-hydroxyl group of the ribose moiety in a ribonucleotide is dependent on NADPH, but this...
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عنوان ژورنال:
- Biochemical Society transactions
دوره 16 2 شماره
صفحات -
تاریخ انتشار 1988